Vaccine development against the Taenia solium parasite: the role of recombinant protein expression in Escherichia coli.

Taenia solium is a parasitic zoonosis that causes cysticercosis. This parasite is a major cause of human illness in poor communities where transmitted to humans from pigs which act as intermediate hosts. Vaccination of pigs to prevent the transmission of T. solium to humans is an approach that has been investigated for controlling this disease. General Recombinant Proteins

A recombinant vaccine antigens, TSOL18, has been very successful in reducing infection with T. solium pigs in tests of experimental challenges. The vaccine has been proven to remove the natural transmission of T. solium obtained in field trials conducted in Africa. We recently reported that the vaccine was also effective in field trials conducted in Peru.

 The TSOL18 recombinant antigen for each of these trials have been produced by expression in Escherichia coli. Here we discuss the research that has been done on TSOL18 antigens and antigens associated with a focus on the preparation of the recombinant TSOL18 repair method and optimized expression in Escherichia coli.

Immunization with Leishmania infantum recombinant protein cyclophilin 1 confers partial protection against subsequent parasitic infections and generate memory T cells specific.

Control of zoonotic visceral leishmaniosis can be achieved by using some of the available drugs. These drugs present a high toxicity and require longer treatment regimens that complicate adherence to treatment. other control measures are directed to a vector or reservoir is a useful tool to curb the spread of this disease, but the effects are temporary

 A vaccine that is safe, affordable and efficient negotiate long-lasting immunity should be an effective way to control the cost of most zoonotic visceral leishmaniosis. The purpose of this study in characterizing cyclophilin protein 1 from Leishmania Human Recombinant Proteins infantum (LiCyP1) to investigate whether the recombinant LiCyP1 (LirCyP1) capable of protection conferred against infection by evaluating the parasitic loads of decent and certain generation of CD4 (+) and CD8 (+) effector and memory cells T central in a mouse model.

 LiCyP1 present in the cytoplasm of L. infantum amastigotes and promastigotes. Immunization of BALB / c mice with LirCyP1 provide high protection against L. infantum infection, cause a marked reduction in parasite replication in the liver and spleen. Furthermore, the helper and cytotoxic T cell subset memory capable of specifically recognizing the parasite antigen expanded in mice immunized and challenged. Subpopulations of CD4 (+) T cells of intermediate phenotype (CD62L (high) CD127 (low)) challenged mice also presented accentuate expansion after the recall. This study shows that LirCyP1 provide partial protection to L.